Identification of Aeromonas hydrophila Cytotoxic Enterotoxin-induced Genes in Macrophages Using Microarrays

Identification of Aeromonas hydrophila cytotoxic enterotoxin-induced genes in macrophages using microarrays.

A cytotoxic enterotoxin (Act) of Aeromonas hydrophila possesses several biological activities, and it induces an inflammatory response in the host. In this study, we used microarrays to gain a global and molecular view of the cellular transcriptional responses to Act and to identify important genes up-regulated by this toxin. Total RNA was isolated at 0, 2, and 12 h from Act-treated macrophages...

The cytotoxic enterotoxin of Aeromonas hydrophila is aerolysin.

The channel-forming toxin aerolysin was identified 25 years ago by Bernheimer and Avigad (1), who gave the protein its name. Aerolysin was purified by Buckley et al. (2), and its structural gene, named aerA, was cloned and sequenced almost simultaneously by two groups (6, 7). Since then more than 50 articles describing the expression, secretion, and properties of aerolysin have appeared, and ae...

Aeromonas hydrophila of Cytotoxic Enterotoxin Alter the Virulence

Microarray analysis of Aeromonas hydrophila cytotoxic enterotoxin-treated murine primary macrophages.

We performed microarray analyses of murine peritoneal macrophages to examine cellular transcriptional responses to a cytotoxic enterotoxin of Aeromonas hydrophila. While 66% of altered genes were common to both primary macrophages and the murine macrophage cell line RAW 264.7, Act caused expression changes of 28 genes specifically in murine peritoneal macrophages.

The cytotoxic enterotoxin of Aeromonas hydrophila induces proinflammatory cytokine production and activates arachidonic acid metabolism in macrophages.

An aerolysin-related cytotoxic enterotoxin (Act) of Aeromonas hydrophila possesses multiple biological activities, which include its ability to lyse red blood cells, destroy tissue culture cell lines, evoke a fluid secretory response in ligated intestinal loop models, and induce lethality in mice. The role of Act in the virulence of the organism has been demonstrated. In this study, we evaluate...